Source

Chinese Hamster Ovary cell line

Description

Human PDGF-BB (Ser82-Thr190)

Accession # P01127

Predicted molecular mass

12.2kDa(monomer)

Specification:

Appearance

White powder, Colorless clear liquid after reconstitution

Purity

≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)

Endotoxin

≤10 EU/mg by the LAL method

Activity

Measured in a cell proliferation assay using NIH3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is ≤10 ng/mL.

Formulation

Lyophilized from a 0.22 μm-filtered solution containing 10mM PB, pH 7.2

Size

1 mg/vial

Handling and Storage:

Reconstitution

It is recommended to redissolve in sterile deionized water.

Shipping

The product is shipped with blue ice

Storage & Stability

36 months at -20°C to -80°C in lyophilized state

6 months at -20°C to -80°C under sterile conditions after reconstitution

7-10 days at 2°C to 8°C under sterile conditions after reconstitution

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Data