KEL Biotech RNA Transfection Reagent Products Help Enable High-Impact Publication in Biomaterials (IF=12.9)

DAT-delivered astaxanthin reprograms adipogenesis through RhoGDI1 dephosphorylation at Ser174 and RhoA/FAK/ERK1/2 cascade suppression

Article Summary:

Soft tissue defects are relatively common in clinical practice, mostly caused by trauma, extensive burns, surgery, and other factors, severely impacting patients' physical and mental health. In plastic surgery and related fields, adipose tissue reconstruction is of great significance for the repair of soft tissue defects [1]. However, constrained by the inherent limitations of current treatment methods, adipose tissue reconstruction still faces critical challenges.

Synthetic materials (fillers and implants) and autologous adipose tissue transplantation (including flap transfer and fat grafting) are currently the primary approaches for repairing soft tissue defects [2]. However, synthetic materials suffer from issues such as easy degradation and poor biocompatibility, while transplanted adipose tissue is prone to calcification, necrosis, and uncontrolled absorption, all of which have raised widespread concern [3].

Recent studies have shown that natural monomers play important roles in adipogenic differentiation and adipose tissue repair [4–6]. Screening natural compounds for monomers with the potential to promote adipogenesis, elucidating their mechanisms of action, and loading them into injectable biohydrogel systems may provide new ideas and directions for soft tissue defect repair.

Astaxanthin (AST), chemically known as 3,3'-dihydroxy-β,β'-carotene-4,4'-dione, has the molecular formula C40H52O4

and a molecular weight of 596.8 Da. Natural astaxanthin is mainly derived from algae, bacteria, yeast, shrimp, trout, crayfish, red yeast, and salmon [7]. The antioxidant activity of astaxanthin is significantly higher than that of common carotenoids [8].

Studies have confirmed that astaxanthin can maintain the normal function of adipose tissue and enhance the activity of adipose-derived stem cells (ADSCs) [9,10]. Astaxanthin exhibits multiple beneficial effects in tissue regeneration, such as promoting angiogenesis, inhibiting extracellular matrix degradation, and exerting anti-inflammatory effects [11–13]. Meanwhile, astaxanthin plays a complex role in cell fate regulation: some studies have reported that it can promote adipogenic differentiation of neural stem cells while inhibiting adipogenic differentiation of 3T3-L1 preadipocytes, displaying a bidirectional regulatory effect [10,14]. However, whether astaxanthin can effectively promote adipose regeneration remains unclear.

In recent years, adipose-derived stem cells (ADSCs) have attracted extensive attention in the field of adipose regeneration due to their strong proliferative capacity and multilineage differentiation potential.

Supporting Products Used in This Study: